Coding

Part:BBa_J92009:Design

Designed by: Timothy Fenton   Group: UC Davis - BIM189C - S10   (2010-05-31)

M. Ruminantium RNA Polymerase Subunit D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 393
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Had to make silent mutations at 306, 648 to get rid of PstI sites.

Source

from Genomic

References